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Image Search Results
Journal: Biophysical Journal
Article Title: Visualizing Tension and Growth in Model Membranes Using Optical Dyes
doi: 10.1016/j.bpj.2018.08.021
Figure Lengend Snippet: Optical probes enable detection of surface area and tension changes using fluorescence microscopy. (a) DOPC vesicles containing membrane dyes are imaged via fluorescence microscopy and show (1) differential interference contrast, (2) Laurdan fluorescence, (3) Cy 5.5 fluorescence, and (4) Cy 7 fluorescence. (b) Representative curve showing that Laurdan GP values decrease as GUVs are exposed to osmotic pressure relative to vesicles exposed to an isotonic solution. (c) FRET between Cy5.5 and Cy7-labeled phospholipids is detected via microscopy. Pseudo-coloring indicates different FRET efficiencies detected across the image. Decreasing FRET values indicate increases in surface area after micelle addition (top). FRET efficiency was averaged across a representative vesicle over time (bottom). Both FRET and Laurdan fluorescence were analyzed in comparison to controls and relative values reflect the difference between GP or FRET values of hypotonically stressed vesicles versus isotonic controls for a representative GUV.
Article Snippet: Chemicals DOPC (18:1 ( Δ 9) 1,2-dioleyoyl-sn-glycero-3-phosphocholine, 25 mg/mL), Cy 5.5 PE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(Cyanine 5.5),
Techniques: Fluorescence, Microscopy, Labeling